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Fluorescence microscopy The plasmid pMCE07 , bearing the C-terminal region of PMP47 fused to GFP, was linearized with MunI prior to transformation into H. The WHS followed one of the largest E. J Antimicrob Chemother 2017 (published online Sep 27) Riedel S. In the QS system, the lasI gene product directs the formation of the diffusible extracellular signal 3-oxo-C12-HSL, and the rhlI product catalyzes the synthesis of C4-HSL ; therefore, the las system positively regulates the expression of rhlI .
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